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      系統將于2020-11-08 09:00 - 10:00 更新維護,給您帶來的不便,敬請諒解!
      Order NO. E607181
      TUNEL 原位細胞凋亡檢測試劑盒,AP
      英文名 In Situ Cell Apoptosis Detection Kit, AP
      相關類別 凋亡檢測 儲存 冷凍(-20℃)
         
      編 號 包裝 庫存 目錄價(¥) 您的價格(¥) 數 量
      E607181-0050 50 TESTS 現貨
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      E607181-0100 100 TESTS 預計5日內發貨
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      產品描述

      概述

      Apoptosis is essential in many physiological processes. Several methods have been described to identify apoptotic cells. Endonucleolysis is considered as the key biochemical event of apoptosis, resulting in double-stranded, low molecular weight DNA fragments as well as single strand breaks in high molecular weight DNA. Those DNA strand breaks can be identified by labeling free 3’-OH termini with modified nucleotides in an enzymatic reaction. Terminal deoxynucleotidyl transferase (TdT) can catalyze DIG-dUTP to free 3’-OH DNA ends. The DIG-dUTP is first react with biotin-conjugated anti-digoxin antibody, which subsequently associate with streptavidin-AP(SABC-AP). Combined with BCIP/NBT Chromogenic Reagent, apoptotic cells stain Purple-blue analyzed under microscopy.

      組份

      Component A: Labeling Buffer; Component B: TdT (20X); Component C: DIG-dUTP (20X); Component D: Blocking Reagent; Component E: Biotin-conjugated anti-digoxin antibody; Component F: SABC-AP (100X); Component G: Protein K (200X); Component H: BCIP/NBT Chromogenic Reagent (20X); Component I: Antibody Dilution; Positive Control

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