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      系統將于2020-11-08 09:00 - 10:00 更新維護,給您帶來的不便,敬請諒解!
      Order NO. E607178
      TUNEL 原位細胞凋亡檢測試劑盒,FITC
      英文名 In Situ Cell Apoptosis Detection Kit, FITC
      相關類別 凋亡檢測 儲存 冷凍(-20℃)
         
      編 號 包裝 庫存 目錄價(¥) 您的價格(¥) 數 量
      E607178-0050 50 TESTS 現貨
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      E607178-0100 100 TESTS 預計5日內發貨
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      產品描述

      概述

      Apoptosis is essential in many physiological processes. Several methods have been described to identify apoptotic cells. Endonucleolysis is considered as the key biochemical event of apoptosis, resulting in double-stranded, low molecular weight DNA fragments as well as single strand breaks in high molecular weight DNA. Those DNA strand breaks can be identified by labeling free 3’-OH termini with modified nucleotides in an enzymatic reaction. Terminal deoxynucleotidyl transferase (TdT) can catalyze DIG-dUTP to free 3’-OH DNA ends. The DIG-dUTP is first react with biotin-conjugated anti-digoxin antibody, which subsequently associate with streptavidin-FITC (SABC-FITC). FITC is excited at around 490-495 nm and emitted visible yellow-green light at 520-530 nm.

      組份

      Component A: Labeling Buffer; Component B: TdT (20X); Component C: DIG-dUTP (20X); Component D: Blocking Reagent; Component E: Biotin-conjugated anti-digoxin antibody; Component F: SABC-FITC (100X); Component G: Protein K (200X); Component H: Antibody Dilution Solution; Positive control samples

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